MYCOTOXIN KITS

Mycotoxins are naturally occurring toxins produced by moulds or fungi in grain while in the field or during storage. Typically triggered by weather extremes, these toxins can cause severe adverse health impacts on people and livestock who consume them. One mycotoxin, aflatoxin, is considered a carcinogen and is strictly regulated by the FDA and EU legislative limits.

Neogen  products offers a comprehensive range of mycotoxin test kits to detect aflatoxin, as well as aflatoxin M1, deoxynivalenol (DON), fumonisin, ochratoxin, T-2/HT-2, and zearalenone, which provide results in minutes while requiring only a minimal amount of training and equipment.

  • Neogen Agri-Screen® natural toxin kits are screening tests that compare up to five samples at a time against a known level of toxin. The tests provide visible results that clearly show whether a sample contains more or less of a toxin than the control provided.
  • Neogen NeoColumn are immunoaffinity columns that efficiently clean and concentrate the toxins prior to analysis by HPLC or Neogen’s Veratox test kits. The Aflatoxin DR column also can be read with a fluorometric reader.
  • Neogen Reveal Q+ (quantitative) kits are extremely easy-to-use and offer rapid and accurate results in minutes.
  • Neogen Reveal Q+ MAX tests feature a common water based extraction that enables users to test 6 different mycotoxins from the same prepared sample. The Reveal Q+ MAX tests are fully quantitative later flow tests offering simple and accurate results in only minutes.
  • Neogen Veratox kits are quantitative tests that compare up to 19 samples at a time against test controls. Through the use of a microwell reader, the tests provide accurate sample results in parts per million (ppm) or parts per billion (ppb).

Neogen’s rapid tests for aflatoxin can screen a wide variety of grain, grain product and nut samples for total aflatoxins (B1, B2, G1, G2) at 20 ppb, or provide fully quantitative results in the range of 1 to 150 ppb.

Aflatoxin is a toxic and carcinogenic substance produced by certain strains of the moulds Aspergillus flavus and A. parasiticus. There are four principal types of aflatoxin in grain and nuts: B1, B2, G1 and G2. Aflatoxin B1 is the most frequently encountered of the group and the most toxic. The commodities most affected by aflatoxin are corn, peanuts, cottonseed.

The commodities most affected by aflatoxin are corn, peanuts, cottonseed, milo, and the majority of tree nuts. When cows consume grain containing aflatoxin, they create a metabolite that is found in their milk, Aflatoxin M1.

Rapid tests for deoxynivalenol (a.k.a. DON and vomitoxin) from Neogen  can screen a wide variety of grain and grain product samples at 1 ppm, or provide fully quantitative results in the range of 25 to 250 ppb (high sensitivity test), or 0.25 to 5 ppm.

Deoxynivalenol (DON) is most commonly produced by the pink mould Fusarium Graminearum (Gibberella Zeae). DON, a member of the trichothecenes family, is produced by fungi living on cereal commodities such as wheat, corn, barley and ensilages. The toxicological effects attributed to DON includes nausea (vomiting), feed refusal, gastroenteritis, diarrhoea, immuno-suppression and blood disorders. Neogen provides several DON (Vomitoxin) testing products.

Rapid tests for fumonisin from Neogen can screen a wide variety of grain and grain product samples at 5 ppm, or provide fully quantitative results in the range of 50 to 600 ppb (high sensitivity test), or 0.5 to 6 ppm.

Fumonisin are a family of mycotoxins produced by the moulds Fusarium moniliforme and F. proliferatum. These moulds commonly infect corn (in fact, they are considered ubiquitous in corn) and rice, hence the potential for fumonisins to be found in feed and foodstuffs is high. Fumonisin affect various animals differently and have been linked to esophageal cancer in humans. The Environmental Protection Agency classifies fumonisins as Category II-B carcinogens.

Horses are extremely sensitive to low amounts of fumonisin, which can cause leukoencephalomalcia (liquefaction of the brain). In swine, research has shown fumonisin attacks the cardiopulmonary system causing pulmonary edema, as well as liver and pancreatic lesions. The FDA has issued guidelines for total fumonisins (FB1+FB2+FB3) in corn and corn by-products in food and animal feeds.

Rapid tests for ochratoxin from Neogen® can provide fully quantitative results for a wide variety of grain, coffee and dried fruit samples in the range of 2 to 25 ppb.

Ochratoxin, commonly produced by the moulds Aspergillus ochraceus and Penicillium viridicatum, can be found in corn, barley, green coffee and various dried fruits. Ochratoxin may be present in conjunction with aflatoxin, one of the most potent naturally-occurring carcinogens. In fact, ochratoxin is a suspected carcinogen. Ochratoxin affects kidneys in animals exposed to naturally-occurring levels of this mycotoxin. Turkeys and other poultry exhibited lower productivity levels during field outbreaks of ochratoxicosis.

Symptoms included retarded growth and decreased feed conversion. It has also been known to affect egg production in laying hens. Although there has been no advisory or regulatory level for ochratoxin issued by the FDA, many agree that levels between 10-20 ppb for commodities destined for human or animal consumption may cause health problems and economic losses. Some foreign markets have set regulation limits ranging from 5 to 50 ppb.

Rapid tests for T-2/HT-2 toxins from Neogen® can provide fully quantitative results for a wide variety of grain and grain product samples in the range of 25-600 ppb.

T-2/HT-2 toxins are trichothecene mycotoxins produced by several species of Fusarium moulds. As T-2 toxin is readily metabolized to HT-2 toxin, and the toxins have been shown to produce numerous adverse effects on many animals, these two mycotoxins are frequently evaluated together. Animals affected by the toxins include swine, dairy cattle, poultry, dogs, cats and horses.

Effects of the toxins include digestive disorders, haemorrhage, edema, oral lesions, dermatitis, and blood disorders. T-2 toxin is the principal causal toxin in the human disease alimentary toxic aleukia. Poultry studies have shown T-2 intoxication has led to a reduction in weight gain and other problems such as beak lesions, poor feathering, motor function impairment and increased susceptibility to Salmonella spp

Rapid tests for zearalenone from Neogen can provide fully quantitative results for a wide variety of grain and grain product samples in the range of 25-1200 ppb.

Zearalenone is primarily produced by the mould Fusarium graminearum, which also commonly produces deoxynivalenol (DON). There is evidence that if zearalenone is detected, there is a high probability that other fusarial mycotoxins may be present. Zearalenone is classified as an estrogenic mycotoxin because it frequently causes estrogenic responses in animals.

When zearalenone-contaminated feed or grain is eaten by livestock, it can cause a wide variety of reproductive problems. In swine, it causes vulvovaginitis, low birth weights, fetal reabsorption, aborted pregnancies, reduced litter sizes, abnormal estrus and feminization of immature males. Zearalenone can delay the breeding process and cost the producer significant economic and physical losses.

Livestock producers are becoming increasingly aware of zearalenone problems, and have looked for ways to reduce risks related to contaminated feed.

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